Malek beat fellow nominees Bradley Cooper (A Star is Born), Willem Dafoe (At Eternity's Gate), Lucas Hedges (Boy Erased) and John David Washington (BlacKkKlansman) for the Best Performance by an Actor in a Motion Picture - Drama award.
"I am beyond moved," he said when he accepted the award, also thanking Freddie Mercury, adding: "This is for and because of you, gorgeous."
Bohemian Rhapsody, in which Malek stars as singer Freddie Mercury, also won the gong for Best Motion Picture - Drama.
To get into the role, not only did Malek get himself a set of fake front teeth (telling Vanity Fair that they 'took some time getting used to'), he also watched Mercury's 1985 Live Aid Performance over 1,500 times on YouTube to get the scene absolutely spot-on.
And he nailed it; every hand gesture, step, head movement and even the pursing of his lips matches up perfectly with Mercury's during the sequence. To be honest, he could have won the Golden Globe for that alone.
Malek arrived on the red carpet earlier this evening with Queen guitarist Brian May, who he was also sat alongside throughout the ceremony - and who's already been busy singing his praises.
A few months ago, May spoke out to say Malek 'deserves an Oscar' for his 'remarkable' performance as Mercury, telling the Press Association: "He's incredible... without doubt he'll be on the nominations list for an Oscar - and well deserved as well."
The 71-year-old musician added: "He inhabited Freddie to the point where we even started to think of him as Freddie. Really remarkable."
The film - which also stars Lucy Boynton, Gwilym Lee, Ben Hardy, Joseph Mazzello, Aidan Gillen, Tom Hollander, Allen Leech and Mike Myers - follows the story of the epic band's rise to fame, quarrels, taking in Freddie's relationship with Mary Austin, his AIDs diagnosis and Queen's iconic Live Aid performance in 1985.
"We've lived with this project for nine years and it's incredible to see it doing so well. It came out to shitty reviews, as some of our work has before, but the public has embraced it magnificently," May added.
"Everybody gave 200 percent, from the actors and technicians to the production team and hair and make-up, everyone. You don't get any lukewarm responses. The guy who plays me should be getting awards all over."
No doubt Malek's Golden Globe win will be one of just many to come - May, keep those fingers crossed for that Oscar, and in the meantime, CONGRATS, RAMI!
Featured Image Credit: 20th Century Fox