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The classic video was uploaded 14 years ago, and to mark the anniversary, the Davis-Carr family are auctioning it off.
The winner will become the sole owner of the video as a non-fungible token (NFT), which is basically a unique unit of data stored on a digital ledger, which can't be exchanged.
But one person's gain will be everyone else's loss, as the video is being taken down from YouTube, where it has been viewed a staggering 882,624,267 times at the time of writing.
In a statement, the family said: "Seen by over 880 million people, Charlie Bit My Finger is the most-viewed viral video of all time.
"The beloved clip has become a household name and holds a special place in the hearts of many.
"Now, the iconic video will be removed from YouTube and one person will have the opportunity to own it in its new form as a 1/1 NFT, memorializing them in internet history forever.
"The NFT winner will also get the opportunity to create their own parody of the video featuring the original stars, Harry and Charlie."
You heard that right, you could get the opportunity to recreate the video with the Harry and Charlie - who now look like this:
The highest bid at the time of writing is $12,500 (£8,833), so you'll have to really want that parody video opportunity. You can bid here.
Speaking about the popularity of the video back in 2009, mum Shelley told The Times: "Susan Boyle has never had the hits we have had.
"The video got on to a college networking site in the US and from there it went viral. I think the British accents have helped make it so globally viewed."
Back then, Shelley had no idea she'd one day sell the vid as an NFT.
NFTs are currently getting a lot of attention, with celebrities and other public figures getting in on the action.
Among those who have decided to cash in on NFTs are Twitter creator Jack Dorsey, who sold the first ever tweet, and Danielle Bregoli - also known as Bhad Bhabie, but probably best known as 'Cash Me Outside' Girl - who auctioned off 20 NFTs to mark her 18th birthday.
Bregoli's NFTs featured original works based on the meme, her music and her rise to viral stardom, as well as works from various creatives including photographers, visual artists and filmmakers.
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