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The beauty and make-up vlogger's husband announced the sad news on Monday, though the cause of death is yet to be made public.
He shared a number of pictures of his wife, as well as a heartfelt message that read: "Mel sadly passed away yesterday.
"It's really hard to just choose 10 photos. We lost a beautiful person."
Thompson had nearly 170,000 subscribers on her channel, as well as 57,000 followers on Instagram.
As well as her make-up tutorials and product reviews, viewers became invested in her life, as she would share regular life updates on her channel as well.
Her final video was shared on September 24.
Mel's husband - named Puffin Thompson - continued: "I just wanted to show the smiles she brought.
"I've had to answer so many texts of people just checking in on her without even knowing of her passing. It's great to see how loved she was. And she loved all you right back.
"I'll continue to love her and miss her forever. She was such a pillar for our family. The kids would talk to her non-stop and she was constantly talking with them and trying to help them with their stuff.
"And no matter how bad she felt, she would still rub my back when I came and jumped on the bed next to her while she was working tirelessly to put out content.
"Everybody knows how talented she was as an artist and with her helpful knowledge, but those closest to her know how truly wonderful she was."
He concluded: "She would have done anything to help anyone without a second thought. I wish I had her back."
Celebrity make-up artist Lisa Eldridge commented on Puffin's Instagram post: "I'm so deeply saddened and shocked.
"My thoughts are with friends and family.... truly heartbreaking. Rest in peace Mel, thank you for always being so wonderful."
Fellow YouTuber Nikkia Joy added: "I am in utter shock. This is the most heartbreaking news. I am so so sorry for your loss, the world will not be the same without Mel."
YouTuber and comedian Jaime French said: "Oh my gosh I am so sorry to hear this and so shocked. She was such a sweet and wonderful, beautiful person. My prayers are with your family."
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