Stylist Love Bailey has claimed Marilyn Manson put a gun to her head during a shoot in 2011.
She made the allegations in an Instagram video - which contains strong language and content some readers may find distressing. You can watch it here:
It is the latest in a string of allegations made against the singer since his former fiancée, actor Evan Rachel Wood, named him as her abuser on Monday (1 February).
Bailey said the incident occurred during a shoot at the 52-year-old's house involving a female actor - the name of whom she censored.
Bailey - who was only 20 at the time - said she noticed 'demonic paintings' on the floor before she was called to Manson's bedroom.
She said: "I crawled over the sex-stained sheets that [the actress] and Marilyn had just finished up whatever they were doing.
"And I looked over to [the actress] and she was black-out wasted. I don't know if this was from pills, she was not coherent at all.
"She was stumbling over, she hurt her knee on the bedside table and Marilyn laughed at her and just let her crawl on the floor.
"So I ran over to help [her], and as I'm holding [her] and trying to get her balance with her, Marilyn pulls a gun to my head. A big Glock, a metal Glock. Not a toy gun - this was a real gun."
While holding the gun to her head, Bailey - who is a trans woman - claimed Manson laughed and said: "I don't like f*****s."
Wood had previously stated she is a survivor of rape and domestic abuse, and earlier this week she named her abuser as Manson.
She said: "He started grooming me when I was a teenager and horrifically abused me for years. I was brainwashed and manipulated into submission.
"I am done living in fear of retaliation, slander, or blackmail. I am here to expose this dangerous man and call out the many industries that have enabled him, before he ruins any more lives.
"I stand with the many victims who will no longer be silent."
Several other women subsequently accused Manson - real name Brian Warner - of abusing them, and he issued a statement denying the allegations.
In a post on Instagram, he said: "Obviously, my art and my life have long been magnets for controversy, but these recent claims about me are horrible distortions of reality.
"My intimate relationships have always been entirely consensual with like-minded partners. Regardless of how - and why - others are now choosing to misrepresent the past, that is the truth."
The singer - whose biggest hit single was a cover of Soft Cell's 'Tainted Love' - has now been dropped by his label.
In a statement to The Hollywood Reporter, Loma Vista Records said: "In light of today's disturbing allegations by Evan Rachel Wood and other women naming Marilyn Manson as their abuser, Loma Vista will cease to further promote his current album, effective immediately.
"Due to these concerning developments, we have also decided not to work with Marilyn Manson on any future projects."
Featured Image Credit: PA
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