Borat was pulled over by police after receiving reports that there was a woman on the roof.
Two cops in Georgia, US, came across the character sitting in a green pickup truck with his 'daughter' strapped to a chair on top.
But despite realising very quickly that it was one of Sacha Baron Cohen's pranks, the officers went along with the gag.
The bizarre encounter, which happened in January, was captured on a police bodycam.
The footage sees Maria Bakalova, 24, portraying the daughter of the Kazakhstan journalist, sitting silently on the roof.
The police were contacted by an anonymous caller who reported the strange sighting.
In the clip, officers Alvey Lamb and Daniel Bride can be seen approaching the car, with Cohen telling them: "I'm new here in this town."
After being asked for some ID, Borat hands officer Bride a piece of paper, and referring to his fake daughter, says: "I have this owner's certificate that proves that she's one of my properties."
As Lamb checks out the registration plate of the motor, Bride asks Cohen to produce something with his name on in English.
When the confused Cohen asks what the matter is, Bride says: "Well, one, you got somebody that's strapped to the top of your car. Do you think that's a little odd?"
The comedian then hands the officer a California driver's licence with the name 'Peter' on it.
Bride asks the joker: "Peter's your first name?"
To which Cohen replies: "Yes."
Eventually, officer Lamb admits that he knew something fishy was going on.
He says: "I'm guessing somebody's around here with a camera, filming, because the dude sitting in the truck looks like Borat, and is talking like Borat, and all that other good stuff. Somebody's goofing off."
Adding: "You know what this is, don't you? Dude, there is a camera around here and I'm not talking about the one on our chest... I didn't sign no waiver or nothing like that. I ain't getting paid for it."
Bride then asks the actor how long the woman is going to stay on the roof, to which he replies: "Officer, I followed the law. I did not feed her an onion. Not that I'm trying to bribe you, but I have a potato here."
The officers tell Cohen that she will have to come down, even if there is no key in the ignition.
A police log of the call reporting the incident said: "Driver is dressed as Borat claiming to be from Uzbekistan. Driver has valid California driver's license doesn't display any mental issues knows date and year. Female sitting on top of vehicle did not speak, appears to be come sort of prank or hidden camera show."
Borat Subsequent Moviefilm is available to watch on Amazon Prime now.