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The protesters wore hazmat bodysuits and tartan face masks, as well as putting up a banner telling visitors to keep out and waving flags and banners.
Eventually, the police were called to the A1 carriageway between Northumberland and Berwickshire to sort the situation out.
The banner raised by the group read: 'Staycation Keep Scotland Covid Free'.
Several videos shared by the protesters have gone viral in the aftermath of the small - and slightly bizarre - event.
In one video, a man can be heard to say: "We are really laying it on thick, and I think what we are saying is, stay the f*** out."
In another clip, a different man said: "We're getting the numbers of caravans and mobile homes that are coming up over the border, getting the numbers and posting them - if they'll not stay at home we'll shame them to death."
It would seem this protest took place because the Scottish Government lifted restrictions that kept people to within five miles of their own house, meaning that those from south of the border can now visit Scotland to their heart's content.
However, Scottish First Minister Nicola Sturgeon has yet to rule out a quarantine period for people from England if the infection rates continue to cause concern.
Up in Scotland, they reported 11 new cases yesterday and no new deaths from the virus.
The protest has been criticised by Berwick's Conservative MP Anne-Marie Trevelyan, who said: "I am dismayed to hear of anyone trying to stop the normal flows of activity which continue day to day across the border.
"This is unacceptable and flies in the face of the realities of the communities around the border for whom daily life and family are border-blind."
While there has been some support for the protests online, a number of Scottish National Party MPs have also criticised the tone of the event.
Glasgow South SNP MP Stewart McDonald wrote: "Nothing but idiots who represent nobody and should be laughed at by everybody. If they had brains they'd be dangerous."
A spokesperson for Police Scotland said: "Police in the Borders were called to a lay-by area near the A1 at Lamberton at around 2.25pm on Saturday, 4 July, 2020 following a report of protest activity at the side of the carriageway.
"Officers attended and suitable advice has been given to those in attendance."
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