The clip was captured just over a week before Halloween on Wednesday 23 October, as a worker at the Lamb and Lion pub cleared tables after breakfast service at around 10.30am.
Assistant manager Lee Smith, 32, said he jumped back in shock after noticing the ghostly figure while checking the CCTV cameras at the pub in York.
While there's obviously no firm proof it was, indeed, an apparition of a 'small girl in a dress', customers have previously reported paranormal activity at the establishment - including hearing a child crying in the room above.
Lee maintains that the footage is unedited, and reckons even the most ardent nonbelievers could be convinced after watching it.
He said: "The camera is close to the bar area and I was round there setting up after breakfast.
"I was checking the CCTV to see where my colleague, Molly was as we were cleaning and preparing for the day ahead.
"I saw the outline of what appeared to be a ghost which caused me to jump back shocked, thinking 'What on earth is that?'
"Molly walked past none the wiser, so I called her and she came and looked at the footage. She was as startled as I was.
"She did say she thought I was in the room with her and felt someone behind her. I don't believe in ghosts but that footage is pretty convincing.
"A lot of people are saying it's the light glaring through the window and someone said it could be cobwebs but there aren't any.
"It looks like a small girl in a dress. It was very strange, especially with Halloween this weekend. A few other colleagues have reported ghost sightings in the past so maybe it's the building."
The Lamb and Lion pub is made up of two Grade II listed buildings.
Number 2 and 2A are understood to have previously been a house and a shop dating back to 1840, while number 4 was a house constructed in 1872, which used to be the Bootham Bar Hotel.
Many locals were feeling shaken by the clip, which the pub posted to Facebook - also asking for the number for 'Ghostbusters'.
One Facebook user commented: "Always thought this place was haunted.
"When I worked as a night porter here at around 2.30/3am all the foot lock doors would shut one by one and would happen once or twice a night."
Someone else wrote: "It always intrigues me how entities can walk through solid objects, and yet don't fall through the floorboards and are able to stand on solid ground."
A third said: "Sounds like the pub should hold an event where people can spend a night there to see if they can see anything."