Tupac - better known by stage name 2Pac - was shot four times whilst sitting in a car driving through Las Vegas alongside his Death Row Records boss Marion 'Suge' Knight.
They'd been watching a fight at the MGM Grand between Mike Tyson and Bruce Seldon, where there was allegedly a confrontation between Pac and his bodyguards and Southside Crips gang member Orlando 'Baby Lane' Anderson.
According to former LAPD Police Department detective Greg Kading, who investigated the murders of both Tupac and fellow rapper Biggie Smalls, that incident was when Shakur 'signed his own death warrant'.
Anderson quickly became one of the main suspects in Shakur's assassination, but to this day nothing has been proven.
That was on September 7 1996. Six days later, Tupac was dead.
After his investigation, Kading said: "When he ran across the lobby of that casino and slugged another gang member, he signed his own death warrant,"
He is also adamant that the fatal shots were fired by Anderson, who was alleged to have been a passenger in the white Cadillac from which they emanated.
However, others have different theories.
Journalist Chuck Philips has claimed since that the assassination took place at the behest of Biggie Smalls, real name Christopher Wallace, who is alleged to have given the assailants the gun and ordered the hit.
He famously offered the Crips $1 million to bump Shakur off before he was eventually killed.
The pair had a long-standing feud that sparked a number of diss tracks, including 2Pac's 'Hit 'Em Up' and Biggie's 'Who Shot Ya?'.
In the aftermath of Tupac's death, Biggie was himself gunned down in another hit, this time allegedly ordered by Knight, who was injured during the Las Vegas shooting that killed Tupac.
Biggie was killed by hitman Wardell 'Poochie' Fouse, but neither he or Anderson ever faced any charges, because they died themselves.
Anderson was killed whilst allegedly collecting a drug debt in 1998, then 'Poochie' was killed by gunfire whilst he was out riding his motorbike in 2003.
That means that both murders are technically still unsolved, and potentially explains why the conspiracy theories continue to circle, and why the case of both artists - tragically taken too soon - continues to be of such interest, even after 25 years.