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The late actor can be seen looking dapper in a tuxedo, performing blues music with Viola Davis.
Netflix shared the trailer to its official Twitter account, with the caption: "It would be an empty world without the Blues.
"Here's Viola Davis and Chadwick Boseman in MA RAINEY'S BLACK BOTTOM, from playwright August Wilson, director George C. Wolfe and producer Denzel Washington.
"On Netflix globally December 18."
The film is based on August Wilson's 1982 play of the same name. It tells the story of Ma Rainey - a pioneer of the blues genre.
Portrayed by Davis, an award-winning actor and producer in her own right, Ma Rainey grapples with issues in 1920s Chicago including racism, relationships and exploitation of Black musicians.
Boseman portrays Ma's boyfriend Levee - a trumpet player who wants to make his own mark on the blues scene in the city.
The film will be out on Netflix just before Christmas.
It has proved to be the final film to feature Boseman, who tragically passed away aged just 43 at the end of August.
In a statement posted to his Twitter, his family revealed the actor had been diagnosed with stage III colon cancer in 2016. He'd been battling with the illness for the last four years as it progressed to stage IV.
He never spoke publicly about his diagnosis and worked through his treatment for much of his career.
He is survived by his wife Taylor.
Boseman managed to shoot and star in several films while undergoing numerous surgeries and chemotherapy.
He's best known for playing King T'Challa in Black Panther as well as other films in the Marvel Cinematic Universe, but recently starred in Spike Lee's Da 5 Bloods.
The 43-year-old was set to star in Black Panther 2, which was due to be released in 2022. The first film, released in 2018, was hugely successful at the box office and received seven Oscar nominations including Best Motion Picture.
He also had prominent roles in Captain America: Civil War, Avengers: Infinity War and Avengers: Endgame. Boseman was beloved by fans for bringing the Wakandan king to life on the big screen.
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